Asian sea bass (Lates calcarifer): Kidney (SISK) and Spleen (SISS) Cell Lines

Applications: Virus isolation and propagation. Toxicological and Gene expression studies.

Deposited at: CAHC Repository, Melvishram and NBFGR, Lucknow, India

Species: Asian sea bass (Lates calcarifer)

Special Characters:

  • Optimum temperature     -    28°C
  • Chromosomal number     -    48 (2n) (Carrey and Mather, 1999)
  • Transfection efficiency    -    High
  • Authentication                 -     By PCR using primer sets of microsatellite markers of sea bass

    1.Sahul Hameed, A.S., Parameswaran, V., Ravi Sukla, Singh, B.  and Ramesh Bhonde, 2006. Establishment and characterization of India’s first marine fish cell line (SISK) from kidney of sea bass, (Lates calcarifer). Aquaculture, 257, 92-103.

   2. Parameswaran, V., Ravi Shukla, Bhonde, R.R. and Sahul Hameed A.S. 2006. Splenic cell line from sea bass, Lates calcarifer: Establishment and characterization. Aquaculture, 261, 43-53.

Developed by
Dr.A.S. Sahul Hameed, C. Abdul Hakeem College, Melvisharam – 632 509 Tamil Nadu, India.
Funded by Department of Biotechnology, Govt. of India.